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Statements Author contributions TA conceptualized the manuscript

The complete absence of dimerization in C396S suggests that Cys396 may be involved in an inter-molecular disulfide bond that results in the formation of an oligomeric structure that combines GSAAT with other enzymes, factors or molecular chaperones involved in ALA formation, such as GluTR, GBP ( The purified UT recombinant GSAAT variants were not entirely reduced, and additional reducing power enhanced their enzyme activities

Stifness and muscle pain can have other causes so it might be something that's worth discussing with your GP, especially if they're getting worse

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Supplemental Figure 3B, C

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